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ISBN: 9781607614609

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Met… Altro …

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2010, ISBN: 9781607614609

Editor: Marelli-Berg, Federica M. Editor: Nourshargh, Sussan, Humana, Hardcover, Auflage: 2010, 302 Seiten, Publiziert: 2010-09-17T00:00:01Z, Produktgruppe: Book, Hersteller-Nr.: 9151223,… Altro …

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T-Cell Trafficking: Methods and Protocols - Federica M. Marelli-Berg
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T-Cell Trafficking: Methods and Protocols - copertina rigida, flessible

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ISBN: 160761460X

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T-Cell Trafficking: Methods and Protocols (Methods in Molecular Biology, 616) - copertina rigida, flessible

2010, ISBN: 160761460X

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T-Cell Trafficking: Methods and Protocols (Methods in Molecular Biology, 616)
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9781607614609


In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.

Informazioni dettagliate del libro - T-Cell Trafficking: Methods and Protocols (Methods in Molecular Biology, 616)


EAN (ISBN-13): 9781607614609
ISBN (ISBN-10): 160761460X
Copertina rigida
Anno di pubblicazione: 2010
Editore: Humana
290 Pagine
Peso: 0,735 kg
Lingua: eng/Englisch

Libro nella banca dati dal 2008-09-01T16:31:27+02:00 (Rome)
Pagina di dettaglio ultima modifica in 2024-02-29T17:12:54+01:00 (Rome)
ISBN/EAN: 9781607614609

ISBN - Stili di scrittura alternativi:
1-60761-460-X, 978-1-60761-460-9
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : berg, marelli, federica, sussan
Titolo del libro: methods cell biology, trafficking, cell and molecular biology, files

Dati dell'editore

Autore: Federica M. Marelli-Berg; Sussan Nourshargh
Titolo: Methods in Molecular Biology; T-Cell Trafficking - Methods and Protocols
Editore: Humana; Humana Press
290 Pagine
Anno di pubblicazione: 2010-09-17
Totowa; NJ; US
Stampato / Fatto in
Lingua: Inglese
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
POD
XII, 290 p. With online files/update.

BB; Hardcover, Softcover / Medizin/Nichtklinische Fächer; Immunologie; Verstehen; Antigen; Fugetaxis; Imaging techniques; Laboratory; Lymphocyte migration; Migratory mechanisms; Multiphoton microscopy; T cell-endothelial cell interactions; molecular biology; molecular mechanisms; Immunology; Biomedical Research; Medizinische Forschung; EA; BC

Introductory Review.- How T Cells Find Their Way Around.- Migration of T Cells In Vitro.- Live Imaging of Leukocyte–Endothelium Interactions.- Leucocyte Adhesion Under Haemodynamic Flow Conditions.- Influence of Stromal Cells on Lymphocyte Adhesion and Migration on Endothelial Cells.- Discriminating Between the Paracellular and Transcellular Routes of Diapedesis.- Monitoring RhoGTPase Activity in Lymphocytes.- Visualisation of Signalling in Immune Cells.- Methods for Quantitation of Leukocyte Chemotaxis and Fugetaxis.- Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes.- Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells.- Migration of T Cells In Vivo.- Visualisation of Lymphoid Organ Development.- Single-Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy.- Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy.- Applying an Adaptive Watershed to the Tissue Cell Quantification During T-Cell Migration and Embryonic Development.- Monitoring T-Cell Migration in Human Diseases.- Identifying Homing Interactions in T-Cell Traffic in Human Disease.- Tracking Antigen-Experienced Effector T Cells In Vitro and In Vivo.- Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo.
Includes detailed description of cutting edge imaging techniques, including multiphoton microscopy, and software-based analysis of T cell infiltration in vivo Describes methods for setting up assays for the observation of lymphocyte migration under flow in vitro Provides description of effective genetic manipulation of the endothelium Includes assays for the investigation of recently described migratory mechanisms, including fugetaxis
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