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Heterologous Gene Expression in E.coli - copertina rigida, flessible

2010, ISBN: 9781617379666

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvemen… Altro …

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Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - copertina rigida, flessible

2010, ISBN: 1617379662

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Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - copertina rigida, flessible

2010

ISBN: 9781617379666

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Jr. Evans, Thomas C.; Ming-Qun Xu:
Heterologous Gene Expression in E.coli - copertina rigida, flessible

2010, ISBN: 9781617379666

Methods and Protocols, Buch, Hardcover, 2011, [PU: Humana Press Inc.], Humana Press Inc., 2010

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Heterologous Gene Expression in E.coli
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Titolo:
ISBN:

1617379662


Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology(TM) format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Informazioni dettagliate del libro - Heterologous Gene Expression in E.coli


EAN (ISBN-13): 9781617379666
ISBN (ISBN-10): 1617379662
Copertina rigida
Anno di pubblicazione: 2011
Editore: Humana Press Inc.
307 Pagine
Peso: 0,758 kg
Lingua: eng/Englisch

Libro nella banca dati dal 2007-10-23T23:28:59+02:00 (Rome)
Pagina di dettaglio ultima modifica in 2023-07-15T15:04:15+02:00 (Rome)
ISBN/EAN: 1617379662

ISBN - Stili di scrittura alternativi:
1-61737-966-2, 978-1-61737-966-6
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : ming, thomas christopher, thomas evans, qun, chris thomas
Titolo del libro: expression, ming, qun

Dati dell'editore

Autore: Thomas C. Evans, Jr.; Ming-Qun Xu
Titolo: Methods in Molecular Biology; Heterologous Gene Expression in E.coli - Methods and Protocols
Editore: Humana; Humana Press
310 Pagine
Anno di pubblicazione: 2010-12-02
Totowa; NJ; US
Stampato / Fatto in
Lingua: Inglese
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
POD
XI, 310 p.

BB; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; Amino acid residues or moieties; Binding partners; Escherichia coli; Expression hosts; Membrane proteins; Post-translational modifications; Protein expression; Recombinant DNA; Recombinant proteins; Proteomics; Medical Genetics; Protein Biochemistry; Biochemie; EA; BC

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Helps to guide scientists toward an ideal expression host Provides easy to follow protocols, ready to be implemented in the lab Includes tips from the experienced, expert contributors on troubleshooting common methodological pitfalls Includes supplementary material: sn.pub/extras
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