ISBN: 9781627031127
The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fasc… Altro …
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2012, ISBN: 162703112X
2012 Gebundene Ausgabe Chemie, Genetik / Humangenetik, Humangenetik, Biochemie, RNApurification; RNAsynthesis; biochemical; biophysical; chemicalsynthesis; gelfiltrationchromatography; … Altro …
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2012, ISBN: 162703112X
2012 Gebundene Ausgabe Chemie, Genetik / Humangenetik, Humangenetik, Genetik, Medizin, RNApurification; RNAsynthesis; biochemical; biophysical; chemicalsynthesis; gelfiltrationchromatog… Altro …
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2012, ISBN: 9781627031127
2012 Buchschnitt verkürzt - gepflegter, sauberer Zustand - Ausgabejahr 2012 22498170/12 Versandkostenfreie Lieferung RNA synthesis,biochemical,in vitro transcription,recombinant approach,… Altro …
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2012, ISBN: 162703112X
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ISBN: 9781627031127
The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fasc… Altro …
Conn, Graeme L. (Herausgeber):
Recombinant and In Vitro RNA Synthesis Methods and Protocols - copertina rigida, flessible2012, ISBN: 162703112X
2012 Gebundene Ausgabe Chemie, Genetik / Humangenetik, Humangenetik, Biochemie, RNApurification; RNAsynthesis; biochemical; biophysical; chemicalsynthesis; gelfiltrationchromatography; … Altro …
2012
ISBN: 162703112X
2012 Gebundene Ausgabe Chemie, Genetik / Humangenetik, Humangenetik, Genetik, Medizin, RNApurification; RNAsynthesis; biochemical; biophysical; chemicalsynthesis; gelfiltrationchromatog… Altro …
2012, ISBN: 9781627031127
2012 Buchschnitt verkürzt - gepflegter, sauberer Zustand - Ausgabejahr 2012 22498170/12 Versandkostenfreie Lieferung RNA synthesis,biochemical,in vitro transcription,recombinant approach,… Altro …
2012, ISBN: 162703112X
2012 Gebundene Ausgabe Chemie, Genetik / Humangenetik, Humangenetik, Biochemie, RNApurification; RNAsynthesis; biochemical; biophysical; chemicalsynthesis; gelfiltrationchromatography; … Altro …
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Informazioni dettagliate del libro - Recombinant and In Vitro RNA Synthesis
EAN (ISBN-13): 9781627031127
ISBN (ISBN-10): 162703112X
Copertina rigida
Anno di pubblicazione: 2012
Editore: Humana Press Inc.
274 Pagine
Peso: 0,703 kg
Lingua: Englisch
Libro nella banca dati dal 2008-05-24T06:03:03+02:00 (Rome)
Pagina di dettaglio ultima modifica in 2023-11-10T20:44:30+01:00 (Rome)
ISBN/EAN: 162703112X
ISBN - Stili di scrittura alternativi:
1-62703-112-X, 978-1-62703-112-7
Stili di scrittura alternativi e concetti di ricerca simili:
Autore del libro : graeme, conn
Titolo del libro: synthesis, vitro, molecular biology rna
Dati dell'editore
Autore: Graeme L. Conn
Titolo: Methods in Molecular Biology; Recombinant and In Vitro RNA Synthesis - Methods and Protocols
Editore: Humana; Humana Press
274 Pagine
Anno di pubblicazione: 2012-10-12
Totowa; NJ; US
Stampato / Fatto in
Peso: 0,773 kg
Lingua: Inglese
106,99 € (DE)
109,99 € (AT)
118,00 CHF (CH)
POD
XIV, 274 p.
BB; Human Genetics; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; RNA purification; RNA synthesis; biochemical; biophysical; chemical synthesis; gel filtration chromatography; in vitro transcription; recombinant approach; recursive PCR protocol; ribozymes; Nucleic Acid Chemistry; Medical Genetics; Nucleic Acid; Technische Anwendung von Biomaterialien; EA; BC
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
aids scientists in continuing to study the fascinating world of RNA.
Recombinant and In Vitro RNA Synthesis: Methods and Protocols Methods in Molecular Biology™ Recombinant and In Vitro RNA Synthesis: Methods and Protocols1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold
Luc Ponchon and Frédéric Dardel
2. Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte and Frédéric Dardel
3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR
Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams
4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE
Jo L. Linpinsel and Graeme L. Conn
5. Preparation of Short RNA by in vitro Transcription
Cheng Lu and Pingwei Li
6. Native RNA Purification by Gel Filtration Chromatography
Evan P. Booy, Hui Meng and Sean A. McKenna
7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends
Johanna M. Avis, Graeme L. Conn, and Scott C. Walker
8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends
Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka
9. Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods
Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong
10. Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA
Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault
11. Affinity Purification of RNA using an ARiBo Tag
Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompré-Brodeur, Vanessa Delfosse and Pascale Legault
12. Plasmid Template Design and in vitro Transcription of Short RNAs within a ‘Structure Cassette’ for Structure Probing Experiments
Virginia K. Vachon and Graeme L. Conn
13. In Vitro Transcription of Modified RNAs
Stephanie L. Moon and Jeffrey Wilusz
14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis
N. Ruth Zearfoss and Sean P. Ryder
15. High-Purity Enzymatic Synthesis of Site-Specifically Modified tRNA
Ya-Ming Hou
16. Se-derivatized RNAs for X-ray Crystallography
Lina Lin and Zhen Huang
17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs
Luigi Martino and Maria R Conte
18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC
Pierre Dagenais and Pascale Legault
19. Splint Ligation of RNA with T4 DNA Ligase
Christopher J. Kershaw and Raymond T. O’Keefe
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
aids scientists in continuing to study the fascinating world of RNA.
Recombinant and In Vitro RNA Synthesis: Methods and Protocols Methods in Molecular Biology™ Recombinant and In Vitro RNA Synthesis: Methods and ProtocolsDetails a collection of protocols that guide the reader from the start of a new RNA project to the final sample
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Includes supplementary material: sn.pub/extras
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